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  • This metadata record represents the R phytoclass package. Determine the chlorophyll a (Chl a) biomass of different phytoplankton groups based on their pigment biomarkers. The method uses non-negative matrix factorisation and simulated annealing to minimise error between the observed and estimated values of pigment concentrations (Hayward et al. (2023) https://doi.org/10.1002/lom3.10541). The approach is similar to the widely used 'CHEMTAX' program (Mackey et al. 1996) https://doi.org/10.3354/meps144265), but is more straightforward, accurate, and not reliant on initial guesses for the pigment to Chl a ratios for each phytoplankton group. Further details are provided at: Hayward, A., M. H. Pinkerton, and A. Gutierrez-Rodriguez. 2023. phytoclass: A pigment-based chemotaxonomic method to determine the biomass of phytoplankton classes. Limnol. Oceanogr. Methods 21: 220–241. https://doi.org/10.1002/lom3.10541 GET PACKAGE: https://cran.r-project.org/web/packages/phytoclass/readme/README.html